However, a small molecule inhibitor of selected estrogen coregulators increased the response of individual alleles to hormone, establishing a previously unrecognized mode of regulation of estro-gen-induced gene activation at the single cell level.
"Estrogen is a type of steroid hormone that modulates a large number of biological functions, both in males and females, by regulating the activity of hundreds of genes per cell," said first au-thor Dr. Fabio Stossi, associate professor of Molecular and Cellular Biology and technical direc-tor of the Integrated Microscopy Core at Baylor.
Deal - Effects - Transcription - Factor - Receptor
A great deal is known about how estrogen triggers its effects. It binds to a nuclear transcription factor (estrogen receptor, or ER), which in turn interacts with specific DNA sequences facilitating the recruitment of coregulators that participate in the regulation of gene expression. It was as-sumed that this process would likely happen simultaneously in all the ER-containing cells in a population that was stimulated with estrogen, but little was known of how actual single cells or individual copies of the same gene responded. Which is why researchers did not anticipate these finding at the single cell level.
"In the current study, we worked with human breast cancer cell lines grown in the lab. Using both molecular and imaging analyses, we determined, at single cell and allele levels, the expres-sion of two well-characterized genes, GREB1 and MYC, whose activity is regulated by estro-gen," said corresponding author, Dr. Michael A. Mancini, professor of Molecular and Cellular Bi-ology, and Pharmacology and Chemical Biology at Baylor. Mancini is also the academic director of the long-running Integrated Microscopy Core (IMC) at Baylor and director of the recently-formed GCC Center for Advanced Microscopy and Image Informatics (CAMII), a CPRIT-funded resource across Baylor and the Texas A&M Institute for Bioscience and Technology.
Researchers - Cells - Lab - Estrogen
The researchers incubated the cells in the lab and treated them with estrogen....
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