Researchers find a way to use CRISPR Cas-9 on reptiles

phys.org | 4/4/2019 | Staff
darktm22darktm22 (Posted by) Level 4
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A team of researchers at the University of Georgia has found a way to use the CRISPR-Cas9 gene editing tool on reptiles. In their paper uploaded to the bioRxiv preprint server, the group describes the technique they developed and how well it worked on test lizards.

CRISPR Cas-9 has been used in a wide variety of experiments over the past couple of decades as scientists attempt to learn more about the procedure itself, how well it works, and possible applications for its use. But early on, researchers suggested that CRISPR could not be used with reptiles because of their unique reproductive systems—anole females, for example, store sperm for use when it is most convenient, making it difficult if not impossible to time the injection of the CRISPR Cas-9 tool. There is also the problem of inserting a needle through an eggshell without damaging it and preventing the embryo from developing. But difficult does not mean impossible, as the team in Georgia has found—they developed a successful workaround to edit cells...
(Excerpt) Read more at: phys.org
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