Gold nano-stars for intracellular delivery

phys.org | 2/21/2019 | Staff
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A new intracellular delivery system. Credit: Timofey Pylaev et al.

Researchers from the Russian Academy of Sciences developed a new method for star-shaped nanoparticles synthesis based on laser irradiation. A wide range of customizable conditions provides an opportunity to create a comfortable environment for various substances for delivery to different types of cells. The results are published in Journal of Biophotonics. The research was supported by the Russian Science Foundation (RNF).

Delivery - Systems - Importance - Research - Techniques

Intracellular delivery systems are of great importance for clinical and laboratory biomedical research. The latest techniques based on viral agents, chemical exposure and microinjections are aimed at achieving maximum efficiency while ensuring high cell viability. However, none of the currently known methods fully satisfies such requirements as compatibility with various cell types and delivered objects, minimal toxicity, maximum efficiency, relative cheapness and simplicity of execution.

The authors of the new study developed a new delivery method using gold nano-stars: star-shaped nanoparticles with sharp spikes. The researchers obtained them by reducing gold ions on spherical embryos of the same metal. After that the nano-stars were deposited in the form of single layers on the plastic surface and covered with cells. Laser irradiation caused electromagnetic waves to travel on the nanoparticles surface, thus transporting substances into the cell.

Scientists - PGFP - DNA - Gene - Protein

Scientists used pGFP (circular DNA with a gene encoding a fluorescent protein) to test the effectiveness of the developed method. They aimed at delivering this molecules into HeLa cells: human cervical cancer lines. This combination of model cells and the delivered object was chosen due to the frequent use of HeLa cells in clinical and biochemical studies,...
(Excerpt) Read more at: phys.org
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