Developing a 3-D collagen model to test magnetic-assisted osteogenesis in vitro

phys.org | 11/12/2018 | Staff
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The cellular and molecular mechanisms of magnetic stimulation-based bone regeneration require further understanding at present. To evaluate the phenomenon in the lab, a three-dimensional (3-D) native collagen model was recently developed via plastic compression to produce a cellular, dense and mechanically strong collagen structure. To produce cell-laden models in the study, Zhiyu Yuan and colleagues incorporated osteoblast cells (MG-63 cell line) and magnetic iron oxide nanoparticles (IONPs) into the collagen gels. Using 3-D printing, a magnetic bioreactor was designed and fabricated to support cell growth under static magnetic fields (SMFs). Using polymerase chain reaction (PCR), the researchers determined the impact of SMFs on the regulation and expression of genes related to osteogenesis including runt-related transcription factor 2 (Runx2), osteonectin (ON) and bone morphogenetic proteins 2 and 4 (BMP-2 and BMP-4).

Now published in Scientific Reports, the results demonstrated that SMFs, IONPs and the collagen matrix were able to stimulate the proliferation, alkaline phosphatase production and mineralization of osteoblasts. The process was enabled by influencing matrix-cell interactions to influence the expression of Runx2, ON, BMP-2 and BMP-4. The collagen model offered insight to progressively form a novel mineralized 3-D bone model and understand magnetic stimulation on osteogenesis. Additional studies can be conducted with the model for applications in tissue engineering and regenerative medicine.

UK - Number - Patients - Bone - Fracture

In the U.K. alone, the number of patients suffering from bone fracture have a substantial economic impact on the quality of life as evidenced with costs to the National Health Services (NHS). During bone injury, the biological and mechanical process of physiological regeneration replaces the injured bone with new bone at the site of injury. The metabolic process is complicated and requires the interaction of many factors, including growth and differentiation factors such as hormones, cytokines and extracellular components; meanwhile inadequate or interrupted factors can lead to delayed healing or...
(Excerpt) Read more at: phys.org
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