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A team of researchers affiliated with several institutions in the U.S. has developed a way to use the CRISPR gene editing technique for dose-dependent activation of gene expressions. In their paper published in the journal Nature Biotechnology, the group describes their technique for altering gene expression in an alternative way and how well it worked.
As the researchers note, prior research has shown that gene expression can be suppressed or activated using the CRISPR/Cas9 gene editing technique. But until now, it had not been used as a way to enable dose-dependent activation of gene expression. In this new effort, the researchers have found a way to do just that by making use of chemical epigenetic modifiers (CEMs) that were specifically designed to activate desired gene expression by using some parts of the endogenous chromatin-activating machinery. Doing so, they note, removed the necessity of exogenous transcriptional activators.
Approach - Components - Cas9 - Protein - FKBP
The new approach involved using two components; the first was Cas9, which was catalytically inactive along with the binding protein FKBP. The second was a CEM made with FK506, which was linked to a molecule that would interact with the desired cellular epigenetic machinery. More...
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